Journal article
Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames
DJ Zanker, S Oveissi, DC Tscharke, M Duan, S Wan, X Zhang, K Xiao, NA Mifsud, J Gibbs, L Izzard, D Dlugolenski, P Faou, KL Laurie, N Vigneron, IG Barr, J Stambas, BJ Van Den Eynde, JR Bennink, JW Yewdell, W Chen
Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2019
Abstract
The importance of antiviral CD8+ T cell recognition of alternative reading frame (ARF)-derived peptides is uncertain. In this study, we describe an epitope (NS1-ARF21-8) present in a predicted 14-residue peptide encoded by the +1 register of NS1 mRNA in the influenza A virus (IAV). NS1-ARF21-8 elicits a robust, highly functional CD8+ T cell response in IAV-infected BALB/c mice. NS1-ARF21-8 is presented from unspliced NS mRNA, likely from downstream initiation on a Met residue that comprises the P1 position of NS1-ARF21-8. Derived from a 14-residue peptide with no apparent biological function and negligible impacts on IAV infection, infectivity, and pathogenicity, NS1-ARF21-8 provides a clear..
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Grants
Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This work was partly supported by National Health and Medical Research Council (NHMRC) Project Grants 433608 and 542508, NHMRC Senior Research Fellowship 603104 to W.C., and NHMRC Program Grant 567122. D.J.Z. was supported by an NHMRC Biomedical Postgraduate scholarship.